Monitoring the ex vivo activation of human T lymphocytes is key to developing an optimized and scalable adoptive cell therapy process. Described here is the development of a large-scale, multiplexed assay using high throughput flow cytometry to assess T-cell activation over time by combining immunophenotyping, cell proliferation, and cytokine profiling in a single assay.

To address the need for rapid monitoring of immune cell function, Intellicyt^? has developed an optimized, high content, multiplexed assay using high throughput flow cytometry to measure T-cell activation. The T-Cell Activation Cell and Cytokine Profiling Kit greatly streamlines the traditional workflow by measuring cell phenotype, T-cell activation markers, cell proliferation, cell viability and quantitates secreted cytokines in a single sample using a miniaturized multiwell plate format.

This study highlights the power of the iQue^? Screener PLUS platform to rapidly characterize multiple endpoints and the ForeCyt^? Software to provide high content visualization that reveals actionable insights to drive faster decisions in the development of cell therapies.

Learn more about Sartorius solutions for CAR-T Discovery & Development at www.sartorius.com/car-t-research.